34th Annual Scientific Meeting proceedings

Objectives:

This prospective study aimed to compare the effect of different preparation methods on PRP cellular content in dogs.

Methods:

PRP was prepared from non anticoagulated blood samples collected from 62 medium to large dogs (≥15kg) using an ACP®-Arthrex Double Syringe and then centrifugated (1500RPM for 5 minutes). Cell counts were analyzed using IDEXX ProCyte-Dx analyzer to :

- Compare cell content of PRP after single centrifugation, second centrifugation and whole blood (WB);

- Compare cell content in the upper and lower layers of plasma after a single centrifugation.

Results:

A single centrifugation increased mean platelet concentration in PRP by 1.3, up to 2.5 times compared to WB and reduced red blood cells (RBC) by 80%. There was marked variability in white blood cells (WBC) after a single centrifugation.

A second centrifugation significantly reduced RBC (95%) and WBC (87%) in PRP compared to WB, but resulted in a 70% loss of platelets.

No difference was noted in the concentration of platelets and RBC between the layers. However, there was a difference in WBC count; 69% reduction in WBC in upper layer versus WB, compared to 30% reduction in the lower.

Coagulation led to exclusion of 33% of samples.

Conclusions:

A second centrifugation with ACP®-Arthrex kit is detrimental for platelets and reduces PRP quality. 

A single centrifugation is recommended. The upper layer seems ideal for low WBC PRP preparations while the lower layer shoud be kept for high WBC PRP preparations.

The use of an anticoagulant appears essential to prevent coagulation.